Thursday 26 November 2015

Know your water enemy

Know your water enemy
The conversion of ammonia to nitrate is actually a two step process carried out by two different classes of bacteria. The first stage is the oxidation of ammonia (NH3) into nitrite (NO2-) by nitrosifying bacteria including species of Nitrosomonas, nitrosococcus, and Nitrosospira. 
The second stage is the oxidation of nitrate to nitrate (NO3-̊ , in this case by nitrifying bacteria, primarily species of Nitrospira rather than the Nitrobacter species often mentioned in older aquarium books.
These bacteria use ammonia and nitrite as a source of energy, oxidising them to release energy the bacteria use to synthesise their food. It is essentially a similar process to photosynthesis, where plants use light energy to synthesise food, except in this case the bacteria are using chemical “fuel” instead of sunlight.

New tank syndrome occurs whenever the filter is not yet fully colonised by sufficient nitrosifying and nitrifying bacteria to deal with the waste produced by the fish in the tank. It isn’t exclusively a problem with new tanks, despite the name. every time you add new fish to a tank, the tanks enter another period of new tank syndrome while the filter bacteria multiply up to deal with increased amounts of fish waste. 
Cleaning the filter media too harshly or careless use of anti-microbial medications will knock back the populations of bacteria as well, setting your aquarium up for another round of new tank syndrome.  

The symptoms
Fish react to new tank syndrome in remarkably consistent ways. Initially, they adopt stress colouration, and behave nervously without showing much interest in food. Air-breathing fish such as gouramis and cordoras make frequent trips to the surface to gulp air, while other will exhibit signs of laboured breathing, such as gasping at the surface or very rapid ventilation of the gills. Cichlids often dart about frantically, while bards tend to adopt odd swimming postures.
If conditions remain poor, the fish start to lose condition rapidly, becoming subject to opportunistic infections such as whitespot, finrot, and fungus. Eventually, the fish die from ammonia or nitrite poisoning. For inexperienced aquarists unaware of the early warning signs, it is usually the sudden death pf most of their fishes that finally sounds the alarm that something isn’t quite right…

Stage one: the ammonia spike
When brand new aquarium is set up, it passes through three distinct stages. The first of these is a rise and fall in the concentration of ammonia, known as the ammonia spike. Ammonia is extremely toxic to fish, with as little as 0.5mg/L being potentially deadly to many species. Once the nitrosifying bacteria get established, the ammonia concentration declines quite rapidly, and often this particular part of the cycling process will be finished within a couple of weeks, at which point the ammonia concentration in the aquarium should be zero.

Stage two: the nitrite spike
A few days after the ammonia concentration has started to decline you should begin to notice a rapid rise in nitrite concentration. This nitrite is of course what the nitrifying bacteria need, and so this is the part of the cycling process where they begin to get established in the filter. Nitrite is marginally less toxic to fish than ammonia, with the critical danger level being around 1.0mg/L. The nitrite spike usually reaches a peak around three to four weeks after the aquarium is set up, after which point the nitrite levels quickly drops down to zero, indicating that the nitrifying bacteria have fully colonised filter.

Stage three : rising nitrate
After about six weeks ammonia and nitrite levels should be zero, and this means that the aquarium has reached the third stage in the cycle, where the filter is fully matured and the only waste product the aquarist has worry about is nitrate isn’t particularly toxic, and levels of 50 to 100mg/L are usually safe for most standard community tropicals. 
Regular water changes are usually enough to dilute the nitrite, keeping the ambient levels low enough for most fish to thrive. However, a few freshwater species are sufficiently intolerant of nitrite that the concentration will need to be kept below 20mg/L. rift valley cichlids and discus are among the best known of these nitrate-intolerant fishes, but mollies kept in freshwater rather than brackish tanks seem to be similarly sensitive.

Quick fixes
So what do you do if your aquarium is suffering from new tank syndrome and your fish are starting to look unhappy. The first thing to do is a water change. A 50% water change will make a huge difference, and performed daily this can get you through the first dangerous weeks of fish keeping without suffering any fatalities. 
The filter bacteria will not be inconvenienced by this, and the idea that they need high levels of ammonia and nitrite to get establish is erroneous. So, along with your nitrite and ammonia test kits, buy a big bottle of dechlorinator and be ready with your bucket and hosepipe to perform large-scale water changes every day.

It is also important to only use hardy fishes while cycling an aquarium. Goldfish and danios work well in freshwater tanks, and black mollies do the job nicely in brackish water tanks. Hardy barbs, such as rosy bards, also work well. Although guppies have often been recommended for cycling tanks in the past, the rather delicate fancy guppies offered for sale are not really up to this job, though wild-caught guppies (if you can find them) may well be. Particular fishes to avoid are loaches, cichlids, and oddballs such as pufferfish.

One way to jump-start a filter is to seed it with filter media taken from another aquarium. A mature filter (at least six month old) can stand to lose up to 50% of its filter media without major problems. Put the media taken from that filter into the new filter, and hey presto, the cycling process is almost entirely leapfrogged. Of course, this does rather depend on not killing the bacteria while moving them from one filter to the other. Treat the bacteria as you would fish: do not expose them to the air, and don’t subject them to large changes in water chemistry or temperature either.

Another way to jump start a filter os to use products such as Tetra SafeStart and Marineland BioSpira. These products contain cultures of bacteria said to colonise a new filter so quickly that fish can be safely introduced at once. While this sounds almost too good to be true, many aquarists have used them with great success. That said, it would still be wise to perform regular ammonia and nitrite tests for a few weeks after using them, and of course carry out water changes as required.

Fishless cycling
When setting up a tank, the temptation is to add too many fish at once, and this often leads to deaths as some of the fish fail to put up with the ambient levels of ammonia and nitrite. Even if you use just a few hardy species, those fish are still being subjected to some pretty nasty water conditions, and if you don’t want danios or goldfish, what do you do with them after you’ve finished cycling the tank? In recent years fishless cycling has become increasingly favoured as the more humane and flexible approach.


Traditionally, this was done by throwing in bits of meat or seafood and just letting nature take its course. As this stuff decayed, it produced ammonia, and that drove the colonisation of the filter by the bacteria. The more modern approach is to add daily doses of ammonia bought from a pharmacy or chemical supplier. Ammonia is of course highly toxic, and should be kept away from pets and children. The exact amount will need to be worked out by trial an error; add some to the aquarium, measure the concentration with an ammonia test kit, and then either add more ammonia or dilute with de-chlorinated water as required. Once you’ve worked out the amount you need to raise the concentration of ammonia in the aquarium to one to five-mg/L, make a note of this and add a similar dose every day. Each day or two, test with your ammonia and nitrite test kits. With luck, you should see the ammonia level peaking first and then the nitrite level, until eventually both settle down to zero indicating that the cycling process has reached completing. 

New tank syndrome is one of those things every aquarist has to deal with. Fortunately, it isn’t that difficult to manage properly, and by using fishless cycling or jump-starting the filter it can even be side-stepped entirely.


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